Compare Two Kinds of Recurrent MI‑Arrest Oocytes

Yan Jiang¹ · Jing‑chuan Yuan¹ · Ge Song¹ · Xiao‑hua Wu¹

Yan Jiang is a Professor, Gynecologist and Obstetrician, embryologist; Jing-chuan Yuan is a Asst. Professor, embryologist; Ge Song is a Asst. Professor, embryologist; Xiao-hua Wu is a Professor, HOD, Gynecologist.

Yan Jiang 13932117427@163.com

Xiao‑hua Wu

1 The Center for Reproductive Medicine and Infertility, The Fourth Hospital of Shijiazhuang, Shijiazhuang Obstetrics and Gynecology Hospital affiliated to Hebei Medical University, Hebei Key Laboratory of Maternal and Fetal Medicine, Shijiazhuang 050011, Hebei, People’s Republic of China

Keywords : ICSI · Re-ICSI · Recurrent MI-arrest oocytes · Indented ZP

During the past few decades, assisted reproductive technology (ART) is widely practiced throughout the world. The purpose of ovarian stimulation in IVF is to recover mature oocytes at metaphase II (MII) stage which are capable of fertilization either when mixed with sperm or after intracytoplasmic sperm injection (ICSI). However, It is common to obtain metaphase I (MI) or germinal vesicle (GV) oocytes in in vitro fertilization (IVF) after controlled ovarian stimulation (COS) [1]. The immature metaphase I oocytes showed a more structured zona pellucida with smooth or blebbing cumulus cells [2]. Two basic ZP layers—outer with rough spongy appearance and inner with smaller fenestrations and smooth fibrous network—were visible.

The cases previously reported recurrent all MI oocytes were resistant to mature after in vitro maturation (IVM) and no fertilization after ICSI [1, 3–5]. The zona pellucida (ZP) of these MI mature-resistant oocytes was observed to be immature, narrow and fibrous [3].

Other studies reported that all oocytes recurrent presented indented ZP with narrow perivitelline space and significantly low oocyte maturity [6, 7]. Sousa [7] reported that all oocytes from one patient were MI oocytes with indented ZP in two cycles. The aim of the present study was to compare two types of recurrent MI oocytes characterized by narrow perivitelline space according to ZP, and to evaluate the fertilization, embryological characteristics of all MI oocytes after ICSI.

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